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biotinylated goat polyclonal anti gas 6 ab  (R&D Systems)


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    R&D Systems biotinylated goat polyclonal anti gas 6 ab
    Biotinylated Goat Polyclonal Anti Gas 6 Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+goat+polyclonal+anti+human+gas6+antibody/Human+Gas6+Antibody/pm30588607-98-7-13
    Average 93 stars, based on 23 article reviews
    biotinylated goat polyclonal anti gas 6 ab - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: The PPAR-γ antagonist GW9662 elicits differentiation of M2c-like cells and upregulation of the MerTK/Gas6 axis: a key role for PPAR-γ in human macrophage polarization
    Article Snippet: A purified goat polyclonal anti-human Gas6 antibody (R&D Systems) was used for capture.A purified goat polyclonal anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. The plates were developed with 3,3′,5,5′-tetramethylbenzidine substrate.The plates were developed with 3,3′,5,5′-tetramethylbenzidine substrate.

    Article Title: IL-17 Stimulates Differentiation of Human Anti-inflammatory Macrophages and Phagocytosis of Apoptotic Neutrophils in Response to IL-10 and Glucocorticoids
    Article Snippet: Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. Statistical analysis Data are expressed as mean±SEM.Statistical analysis Data are expressed as mean±SEM.

    Article Title: Efficient clearance of early apoptotic cells by human macrophages requires "M2c" polarization and MerTK induction
    Article Snippet: Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. Secretion of IL-10 and TNFα was induced by LPS (50 ng/ml; Sigma-Aldrich) ± rhGas6 (1 μg/ml; R&D Systems), in the presence or absence of goat anti-human MerTK antibody (R&D Systems) or goat control IgG (SouthernBiotech), from cells cultured with or without M-CSF.Secretion of IL-10 and TNFα was induced by LPS (50 ng/ml; Sigma-Aldrich) ± rhGas6 (1 μg/ml; R&D Systems), in the presence or absence of goat anti-human MerTK antibody (R&D Systems) or goat control IgG (SouthernBiotech), from cells cultured with or without M-CSF.

    Expressing:

    Article Title: The PPAR-γ antagonist GW9662 elicits differentiation of M2c-like cells and upregulation of the MerTK/Gas6 axis: a key role for PPAR-γ in human macrophage polarization
    Article Snippet: A purified goat polyclonal anti-human Gas6 antibody (R&D Systems) was used for capture.A purified goat polyclonal anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. The plates were developed with 3,3′,5,5′-tetramethylbenzidine substrate.The plates were developed with 3,3′,5,5′-tetramethylbenzidine substrate.

    Article Title: IL-17 Stimulates Differentiation of Human Anti-inflammatory Macrophages and Phagocytosis of Apoptotic Neutrophils in Response to IL-10 and Glucocorticoids
    Article Snippet: Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. Statistical analysis Data are expressed as mean±SEM.Statistical analysis Data are expressed as mean±SEM.

    Article Title: Efficient clearance of early apoptotic cells by human macrophages requires "M2c" polarization and MerTK induction
    Article Snippet: Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. Secretion of IL-10 and TNFα was induced by LPS (50 ng/ml; Sigma-Aldrich) ± rhGas6 (1 μg/ml; R&D Systems), in the presence or absence of goat anti-human MerTK antibody (R&D Systems) or goat control IgG (SouthernBiotech), from cells cultured with or without M-CSF.Secretion of IL-10 and TNFα was induced by LPS (50 ng/ml; Sigma-Aldrich) ± rhGas6 (1 μg/ml; R&D Systems), in the presence or absence of goat anti-human MerTK antibody (R&D Systems) or goat control IgG (SouthernBiotech), from cells cultured with or without M-CSF.

    Flow Cytometry:

    Article Title: The PPAR-γ antagonist GW9662 elicits differentiation of M2c-like cells and upregulation of the MerTK/Gas6 axis: a key role for PPAR-γ in human macrophage polarization
    Article Snippet: A purified goat polyclonal anti-human Gas6 antibody (R&D Systems) was used for capture.A purified goat polyclonal anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. The plates were developed with 3,3′,5,5′-tetramethylbenzidine substrate.The plates were developed with 3,3′,5,5′-tetramethylbenzidine substrate.

    Article Title: IL-17 Stimulates Differentiation of Human Anti-inflammatory Macrophages and Phagocytosis of Apoptotic Neutrophils in Response to IL-10 and Glucocorticoids
    Article Snippet: Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. Statistical analysis Data are expressed as mean±SEM.Statistical analysis Data are expressed as mean±SEM.

    Article Title: Efficient clearance of early apoptotic cells by human macrophages requires "M2c" polarization and MerTK induction
    Article Snippet: Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. Secretion of IL-10 and TNFα was induced by LPS (50 ng/ml; Sigma-Aldrich) ± rhGas6 (1 μg/ml; R&D Systems), in the presence or absence of goat anti-human MerTK antibody (R&D Systems) or goat control IgG (SouthernBiotech), from cells cultured with or without M-CSF.Secretion of IL-10 and TNFα was induced by LPS (50 ng/ml; Sigma-Aldrich) ± rhGas6 (1 μg/ml; R&D Systems), in the presence or absence of goat anti-human MerTK antibody (R&D Systems) or goat control IgG (SouthernBiotech), from cells cultured with or without M-CSF.

    Enzyme-linked Immunosorbent Assay:

    Article Title: The PPAR-γ antagonist GW9662 elicits differentiation of M2c-like cells and upregulation of the MerTK/Gas6 axis: a key role for PPAR-γ in human macrophage polarization
    Article Snippet: A purified goat polyclonal anti-human Gas6 antibody (R&D Systems) was used for capture.A purified goat polyclonal anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. The plates were developed with 3,3′,5,5′-tetramethylbenzidine substrate.The plates were developed with 3,3′,5,5′-tetramethylbenzidine substrate.

    Article Title: IL-17 Stimulates Differentiation of Human Anti-inflammatory Macrophages and Phagocytosis of Apoptotic Neutrophils in Response to IL-10 and Glucocorticoids
    Article Snippet: Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. Statistical analysis Data are expressed as mean±SEM.Statistical analysis Data are expressed as mean±SEM.

    Article Title: Efficient clearance of early apoptotic cells by human macrophages requires "M2c" polarization and MerTK induction
    Article Snippet: Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. Secretion of IL-10 and TNFα was induced by LPS (50 ng/ml; Sigma-Aldrich) ± rhGas6 (1 μg/ml; R&D Systems), in the presence or absence of goat anti-human MerTK antibody (R&D Systems) or goat control IgG (SouthernBiotech), from cells cultured with or without M-CSF.Secretion of IL-10 and TNFα was induced by LPS (50 ng/ml; Sigma-Aldrich) ± rhGas6 (1 μg/ml; R&D Systems), in the presence or absence of goat anti-human MerTK antibody (R&D Systems) or goat control IgG (SouthernBiotech), from cells cultured with or without M-CSF.

    Incubation:

    Article Title: The PPAR-γ antagonist GW9662 elicits differentiation of M2c-like cells and upregulation of the MerTK/Gas6 axis: a key role for PPAR-γ in human macrophage polarization
    Article Snippet: A purified goat polyclonal anti-human Gas6 antibody (R&D Systems) was used for capture.A purified goat polyclonal anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. The plates were developed with 3,3′,5,5′-tetramethylbenzidine substrate.The plates were developed with 3,3′,5,5′-tetramethylbenzidine substrate.

    Article Title: IL-17 Stimulates Differentiation of Human Anti-inflammatory Macrophages and Phagocytosis of Apoptotic Neutrophils in Response to IL-10 and Glucocorticoids
    Article Snippet: Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. Statistical analysis Data are expressed as mean±SEM.Statistical analysis Data are expressed as mean±SEM.

    Article Title: Efficient clearance of early apoptotic cells by human macrophages requires "M2c" polarization and MerTK induction
    Article Snippet: Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. Secretion of IL-10 and TNFα was induced by LPS (50 ng/ml; Sigma-Aldrich) ± rhGas6 (1 μg/ml; R&D Systems), in the presence or absence of goat anti-human MerTK antibody (R&D Systems) or goat control IgG (SouthernBiotech), from cells cultured with or without M-CSF.Secretion of IL-10 and TNFα was induced by LPS (50 ng/ml; Sigma-Aldrich) ± rhGas6 (1 μg/ml; R&D Systems), in the presence or absence of goat anti-human MerTK antibody (R&D Systems) or goat control IgG (SouthernBiotech), from cells cultured with or without M-CSF.

    Control:

    Article Title: The PPAR-γ antagonist GW9662 elicits differentiation of M2c-like cells and upregulation of the MerTK/Gas6 axis: a key role for PPAR-γ in human macrophage polarization
    Article Snippet: A purified goat polyclonal anti-human Gas6 antibody (R&D Systems) was used for capture.A purified goat polyclonal anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. The plates were developed with 3,3′,5,5′-tetramethylbenzidine substrate.The plates were developed with 3,3′,5,5′-tetramethylbenzidine substrate.

    Article Title: IL-17 Stimulates Differentiation of Human Anti-inflammatory Macrophages and Phagocytosis of Apoptotic Neutrophils in Response to IL-10 and Glucocorticoids
    Article Snippet: Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. Statistical analysis Data are expressed as mean±SEM.Statistical analysis Data are expressed as mean±SEM.

    Article Title: Efficient clearance of early apoptotic cells by human macrophages requires "M2c" polarization and MerTK induction
    Article Snippet: Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.Purified goat anti-human Gas6 antibody (R&D Systems) was used for capture.. Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.. Secretion of IL-10 and TNFα was induced by LPS (50 ng/ml; Sigma-Aldrich) ± rhGas6 (1 μg/ml; R&D Systems), in the presence or absence of goat anti-human MerTK antibody (R&D Systems) or goat control IgG (SouthernBiotech), from cells cultured with or without M-CSF.Secretion of IL-10 and TNFα was induced by LPS (50 ng/ml; Sigma-Aldrich) ± rhGas6 (1 μg/ml; R&D Systems), in the presence or absence of goat anti-human MerTK antibody (R&D Systems) or goat control IgG (SouthernBiotech), from cells cultured with or without M-CSF.



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    R&D Systems biotinylated goat polyclonal anti gas 6 ab
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    GW9662 induces M2c-like cells that upregulate MerTK and its ligand <t>Gas6.</t> (A-C) Healthy monocytes were cultured in serum-free medium in the absence of cytokines or growth factors (M0 differentiation), with or without the PPAR-γ antagonist GW9662 (2.5-10 μM), for 4 days; when specified, the PPAR-γ agonist rosiglitazone (1 μM) was added. Expression of MerTK, CD163 and CD16 was measured by flow cytometry. (D-E) Gas6 production levels were quantified by ELISA in culture medium, upon incubation with or without GW9662 (2.5-10 μM) of otherwise untreated cells (M0 conditions), LPS (50 ng/ml; M1 conditions) or IL-4 (20 ng/ml; M2a conditions) exposed cells. (A-E) Pooled data are represented as mean values ± SEM. Analysis was performed using one-way repeated measures ANOVA with Newman-Keuls multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. When not specified by additional graphic signs, statistical annotations (asterisks) refer to comparisons with respect to the relative GW9662 untreated control group. Each set of data is representative of three independent experiments.
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    R&D Systems biotinylated goat anti human gas6 polyclonal antibody
    GW9662 induces M2c-like cells that upregulate MerTK and its ligand <t>Gas6.</t> (A-C) Healthy monocytes were cultured in serum-free medium in the absence of cytokines or growth factors (M0 differentiation), with or without the PPAR-γ antagonist GW9662 (2.5-10 μM), for 4 days; when specified, the PPAR-γ agonist rosiglitazone (1 μM) was added. Expression of MerTK, CD163 and CD16 was measured by flow cytometry. (D-E) Gas6 production levels were quantified by ELISA in culture medium, upon incubation with or without GW9662 (2.5-10 μM) of otherwise untreated cells (M0 conditions), LPS (50 ng/ml; M1 conditions) or IL-4 (20 ng/ml; M2a conditions) exposed cells. (A-E) Pooled data are represented as mean values ± SEM. Analysis was performed using one-way repeated measures ANOVA with Newman-Keuls multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. When not specified by additional graphic signs, statistical annotations (asterisks) refer to comparisons with respect to the relative GW9662 untreated control group. Each set of data is representative of three independent experiments.
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    GW9662 induces M2c-like cells that upregulate MerTK and its ligand Gas6. (A-C) Healthy monocytes were cultured in serum-free medium in the absence of cytokines or growth factors (M0 differentiation), with or without the PPAR-γ antagonist GW9662 (2.5-10 μM), for 4 days; when specified, the PPAR-γ agonist rosiglitazone (1 μM) was added. Expression of MerTK, CD163 and CD16 was measured by flow cytometry. (D-E) Gas6 production levels were quantified by ELISA in culture medium, upon incubation with or without GW9662 (2.5-10 μM) of otherwise untreated cells (M0 conditions), LPS (50 ng/ml; M1 conditions) or IL-4 (20 ng/ml; M2a conditions) exposed cells. (A-E) Pooled data are represented as mean values ± SEM. Analysis was performed using one-way repeated measures ANOVA with Newman-Keuls multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. When not specified by additional graphic signs, statistical annotations (asterisks) refer to comparisons with respect to the relative GW9662 untreated control group. Each set of data is representative of three independent experiments.

    Journal: Journal of Inflammation (London, England)

    Article Title: The PPAR-γ antagonist GW9662 elicits differentiation of M2c-like cells and upregulation of the MerTK/Gas6 axis: a key role for PPAR-γ in human macrophage polarization

    doi: 10.1186/s12950-015-0081-4

    Figure Lengend Snippet: GW9662 induces M2c-like cells that upregulate MerTK and its ligand Gas6. (A-C) Healthy monocytes were cultured in serum-free medium in the absence of cytokines or growth factors (M0 differentiation), with or without the PPAR-γ antagonist GW9662 (2.5-10 μM), for 4 days; when specified, the PPAR-γ agonist rosiglitazone (1 μM) was added. Expression of MerTK, CD163 and CD16 was measured by flow cytometry. (D-E) Gas6 production levels were quantified by ELISA in culture medium, upon incubation with or without GW9662 (2.5-10 μM) of otherwise untreated cells (M0 conditions), LPS (50 ng/ml; M1 conditions) or IL-4 (20 ng/ml; M2a conditions) exposed cells. (A-E) Pooled data are represented as mean values ± SEM. Analysis was performed using one-way repeated measures ANOVA with Newman-Keuls multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. When not specified by additional graphic signs, statistical annotations (asterisks) refer to comparisons with respect to the relative GW9662 untreated control group. Each set of data is representative of three independent experiments.

    Article Snippet: Biotinylated goat polyclonal anti-human Gas6 antibody (R&D Systems), followed by HRP-conjugated streptavidin (Biolegend), was used for detection.

    Techniques: Cell Culture, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Incubation, Control